SPARC Antibody (C-term)

• Catalog Number: orb1929835
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB19869
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse, Other, Porcine, Rabbit, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 34632 Da
• Target: This SPARC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human SPARC.
• UniProt ID: P09486
• NCBI: NP_003109.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: SPARC, Basement-membrane protein 40, BM-40, Osteon
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of widr cells using SPARC Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human skin tissue reacted with SPARC Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of SPARC Antibody (C-term) in Y79 cell line lysates (35 ug/lane). SPARC (arrow) was detected using the purified Pab.

Western blot analysis of SPARC (arrow) using rabbit polyclonal SPARC Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPARC gene.