SOX9 Antibody

• Catalog Number: orb1268563
• Category: Antibodies
• Description: SOX9 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Mouse, Porcine
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This SOX9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the N-terminal region of human SOX9.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 56 kDa
• Target: SOX9
• UniProt ID: P48436
• NCBI: P48436
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Transcription factor SOX-9, SOX9
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of anti-SOX9 Antibody in K562 cell line lysates (35 ug/lane)

Western blot analysis of SOX9 using rabbit polyclonal SOX9 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX9 gene (Lane 2).

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with SOX9 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Immunofluorescence analysis of anti-SOX9 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H + L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.