SOX2 Antibody

• Catalog Number: orb639557
• Category: Antibodies
• Description: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. [UniProt]
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: SBX2-1R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Amino acids 176-305 were used as the immunogen for the recombinant SOX2 antibody.
• Dilution range: ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant SOX2 antibody is available for research use only.
• UniProt ID: P48431
• Storage: Store the recombinant SOX2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human cervical carcinoma with recombinant SOX2 antibody (clone SBX2-1R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of NCCIT lysate with recombinant SOX2 antibody (clone SBX2-1R). Predicted molecular weight ~34 kDa.