Sonic Hedgehog (SHH) antibody

• Catalog Number: orb1338678
• Category: Antibodies
• Description: Mouse monoclonal antibody to Sonic Hedgehog (SHH)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: OTI3A2
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2a
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 24-197 of human SHH
• Dilution range: WB: 1:2000, IF (1:100)
• Conjugation: Unconjugated
• MW: 27.6 kDa (Actual 22 kDa/55 kDa)
• Target: SHH
• UniProt ID: Q15465
• NCBI: NM_000193
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHH (Right lane) cDNA for 48 hrs and lysed using Sonic Hedgehog (SHH) antibody