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Catalog Number | orb1821646 |
---|---|
Category | Antibodies |
Description | Softag 1 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | NT73 |
Tested applications | ELISA, WB |
Reactivity | E. coli |
Isotype | IgG |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Target | Softag 1 |
UniProt ID | P0A8T7 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | rpoC; tabB; epitope tag (SLAELLNAGLGGS); DNA-direc Read more... |
Note | For research use only |
Application notes | This antibody was initially generated for purification of Escherichia coli RNA polymerase because of its ability to release the RNA polymerase in the presence of a low molecular weight polyhydroxylated compound (polyol) and a non-chaotropic salt. Such antibodies were termed as "polyol responsive" MAbs. Using NT73 conjugated to Sepharose, highly active RNA polymerase could be prepared rapidly by a single immunoaffinity chromatography step, replacing two lengthy chromatographic steps in the conventional purification procedure. The polyol-responsiveness of the antibody was determined by an ELISA-elution assay (PMID: 1637835). Later on the epitope of the abtibody was recognized and found to be a 13-amino-acid sequence 'SLAELLNAGLGGS' which was converted to an epitope tag that can be fused to a protein of interest for use as a purification tag. This epitope tag called 'Softag1' was fused to either the N or the C terminus of the green fluorescent protein. These tagged proteins were expressed in E. coli, and the tagged proteins were purified from the soluble fraction by a single-step immunoaffinity chromatography procedure. This approach extends the powerful technique of gentle-release immunoaffinity chromatography to many expressed proteins (PMID: 14656522). The scFv variants of this antibody loses considerable affinity for its antigen, however it maintains similar polyol-responsiveness as the parent monoclonal antibody (PMID: 16216525). |
Expiration Date | 12 months from date of receipt. |
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