Sodium Potassium ATPase Mouse Monoclonal Antibody

• Catalog Number: orb704302
• Category: Antibodies
• Description: Sodium Potassium ATPase Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2G11
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1,k
• Immunogen: Recombinant human Sodium Potassium ATPase (551-850/1023aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:2000-10000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 113 kDa
• Target: ATP1A1
• UniProt ID: P05023
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ATP1A1; alpha 1 Sodium Potassium ATPase; A1A1; AT1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded Human Fundus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

25 ug total protein per lane of various lysates probed with Sodium Potassium ATPase monoclonal antibody, unconjugated (orb704302) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Sodium Potassium ATPase Monoclonal Antibody, Unconjugated (orb704302) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.