SOCS1 Antibody (N-term)

• Catalog Number: orb1928279
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB17347
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 23551 Da
• Target: This SOCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-66 amino acids from the N-terminal region of human SOCS1.
• UniProt ID: O15524
• NCBI: NP_003736.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Suppressor of cytokine signaling 1, SOCS-1, JAK-bi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SOCS1 Antibody (N-term) in mouse kidney tissue lysates (35 ug/lane). SOCS1 (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of SOCS1 Antibody (N-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

SOCS1 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SOCS1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-SOCS1 Antibody (N-term) at 1:2000 dilution. Lane 1: mouse thymus lysates. Lane 2: rat spleen lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 24 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.