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Catalog Number | orb1929340 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB19144 |
Tested applications | FC, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Dilution range | WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 49097 Da |
Target | This SMYD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-35 amino acids from the N-terminal region of human SMYD3. |
UniProt ID | Q9H7B4 |
NCBI | NP_001161212.1, NP_073580.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Histone-lysine N-methyltransferase SMYD3, SET and Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
SMYD3 Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SMYD3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SMYD3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of SMYD3 antibody (N-term) in CEM cell line lysates (35 ug/lane). SMYD3 (arrow) was detected using the purified Pab.