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Item 1 of 3
SMYD3 Antibody (Center)
Catalog Number: orb1929339
| Catalog Number | orb1929339 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB19145 |
| Tested applications | FC, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | WB: 1:1000, WB: 1:1000, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 49097 Da |
| Target | This SMYD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-236 amino acids from the Central region of human SMYD3. |
| UniProt ID | Q9H7B4 |
| NCBI | NP_001161212.1, NP_073580.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Histone-lysine N-methyltransferase SMYD3, SET and Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
SMYD3 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of SMYD3 antibody (Center) in CEM cell line lysates (35 ug/lane). SMYD3 (arrow) was detected using the purified Pab.
Western blot analysis of lysate from HT-29 cell line, using SMYD3 Antibody (Center). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
