SMYD2 Antibody (N-term)

• Catalog Number: orb1938982
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB14276
• Tested applications: WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, WB: 1:1000, WB: 1:1000, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 49688 Da
• Target: This SMYD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-152 amino acids from the N-terminal region of human SMYD2.
• UniProt ID: Q9NRG4
• NCBI: NP_064582.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: N-lysine methyltransferase SMYD2, 211-, HSKM-B, Hi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-SMYD2 Antibody (N-term) at 1:2000 dilution. Lane 1: HT-1080 whole cell lysates. Lane 2: mouse heart lysates. Lane 3: Hela whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

SMYD2 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35 ug/lane). This demonstrates the SMYD2 antibody detected the SMYD2 protein (arrow).

SMYD2 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35 ug/lane). This demonstrates the SMYD2 antibody detected the SMYD2 protein (arrow).

Western blot analysis of SYMD2 (arrow) using rabbit polyclonal SYMD2 Antibody (N-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SYMD2 gene (Lane 2).