SMURF2 Antibody

• Catalog Number: orb1240007
• Category: Antibodies
• Description: SMURF2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: SMURF2 antibody was raised against a 17 amino acid peptide near the amino terminus of human SMURF2.The immunogen is located within amino acids 140 - 190 of SMURF2.
• Concentration: 1 mg/mL
• Dilution range: SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 82 kDa Observed: 85 kDa
• Target: SMURF2
• UniProt ID: Q9HAU4
• NCBI: NP_073576
• Storage: SMURF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: SMURF2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SMURF2 Antibody: E3 ubiquitin-protein ligase SMURF
• Note: For research use only
• Application notes: SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SMURF2 in C2C12 cell lysate with SMURF2 antibody at 1 µg/ml.

Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 5 µg/mL.

Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20 µg/mL.

Immunofluorescence of SMURF2 in human uterus tissue with SMURF2 antibody at 20 µg/ml. Green: SMURF2 Antibody (orb1240007) Blue: DAPI staining.

Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20 µg/ml. Green: SMURF2 Antibody (orb1240007) Blue: DAPI staining.

Immunohistochemistry of SMURF2 in human uterus tissue with SMURF2 antibody at 2 µg/ml.

Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 2 µg/ml.