α-Smooth Muscle Actin

• Catalog Number: orb1294387
• Category: Antibodies
• Description: Rabbit polyclonal antibody to alpha Smooth Muscle Actin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the center region
• Dilution range: Western Blot 1:1000-1:3000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:400 Immunohistochemistry (Frozen) 1:100-1:200
• Conjugation: Unconjugated
• Target: α-Smooth Muscle Actin
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-α Smooth Muscle Actin antibody at 1/2000 dilution, Lysates at 35 μg per lane, This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294387 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling α Smooth Muscle Actin with orb1294387 at 1/300.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling α Smooth Muscle Actin with orb1294387 at 1/300.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling α Smooth Muscle Actin with orb1294387 at 1/200.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling α Smooth Muscle Actin with orb1294387 at 1/200.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294387 Anti-α Smooth Muscle Actin antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294387 Anti-α Smooth Muscle Actin antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling α Smooth Muscle Actin with orb1294387 at 1/300.

Frozen sectioned for immunohistochemical analysis. Sample: Frozen section. Red: α Smooth Muscle Actin antibody. Catalog: orb1294387 at 1-200.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling α Smooth Muscle Actin with orb1294387 at 1/200.