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Catalog Number | orb1294387 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to alpha Smooth Muscle Actin |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC-Fr, IHC-P, WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / encompassing a sequence within the center region |
Dilution range | Western Blot 1:1000-1:3000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:400 Immunohistochemistry (Frozen) 1:100-1:200 |
Conjugation | Unconjugated |
Target | α-Smooth Muscle Actin |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-α Smooth Muscle Actin antibody at 1/2000 dilution, Lysates at 35 μg per lane, This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294387 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling α Smooth Muscle Actin with orb1294387 at 1/300.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling α Smooth Muscle Actin with orb1294387 at 1/300.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling α Smooth Muscle Actin with orb1294387 at 1/200.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling α Smooth Muscle Actin with orb1294387 at 1/200.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294387 Anti-α Smooth Muscle Actin antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294387 Anti-α Smooth Muscle Actin antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling α Smooth Muscle Actin with orb1294387 at 1/300.
Frozen sectioned for immunohistochemical analysis. Sample: Frozen section. Red: α Smooth Muscle Actin antibody. Catalog: orb1294387 at 1-200.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling α Smooth Muscle Actin with orb1294387 at 1/200.
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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