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Catalog Number | orb1825322 |
---|---|
Category | Antibodies |
Description | Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | SM-M10 |
Tested applications | FACS, IF, IHC-P |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | Crude human uterus extract was used as the immunogen for the SMMHC antibody. |
Dilution range | Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT |
Conjugation | Unconjugated |
Formula | 1 mg/ml in 1X PBS; BSA free, sodium azide free |
Hazard Information | This SMMHC antibody is available for research use only. |
UniProt ID | P35749 |
Storage | Aliquot the SMMHC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human uterus tissue with SMMHC antibody (clone SM-M10). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free SM-MHC antibody (clone SM-M10) as confirmation of integrity and purity.
IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
IHC-P | |
Human | |
Mouse | |
Recombinant | |
Unconjugated |
IHC-P | |
Human | |
Mouse | |
Recombinant | |
Unconjugated |
IHC-P | |
Human | |
Mouse | |
Recombinant | |
Unconjugated |
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