SMMHC Antibody Cocktail (Smooth Muscle Myosin Heavy Chain)

• Catalog Number: orb749708
• Category: Antibodies
• Description: Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MYH11/923 + SMMS-1
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human protein (MYH11/923) and human uterus extract (SMMS-1) were used as the immunogen for the SMMHC antibody cocktail.
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This SMMHC antibody cocktail is available for research use only.
• UniProt ID: P35749
• Storage: Store the SMMHC antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the SMMHC antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with SMMHC antibody (MYH11/923 + SMMS-1).

IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with SMMHC antibody (MYH11/923 + SMMS-1).

IHC: Formalin-fixed, paraffin-embedded human angiosarcoma stained with SMMHC antibody (MYH11/923 + SMMS-1).

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with SMMHC antibody (MYH11/923 + SMMS-1).