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Catalog Number | orb1522621 |
---|---|
Category | Antibodies |
Description | SMC3, Phospho (S1083) Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | Surrounding serine 1083 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:500 - 1:2,500; IP - 2 - 10 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | SMC3, Phospho (S1083) |
UniProt ID | Q9UQE7 |
NCBI | NP_005436.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | BAM; CSPG6; structural maintenance of chromosomes Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human Phospho SMC3 (S1083) by western blot (WB). Samples: Whole cell lysate (50 µg) from HeLa cells treated with 100 µM etoposide and/or 100 µM phosphatase for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody orb1522621 used for WB at 1 µg/ml (upper panel) ; to detect total SMC3, rabbit anti-SMC3 was used for WB at 0.1 µg/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) or 10 seconds (lower panel).
Detection of human Phospho SMC3 (S1083) by western blot of immunoprecipitates. Samples: Whole cell lysate (1mg for IP; 20% of IP loaded) from HeLa cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-). Antibodies: Phospho SMC3 (S1083) was immunoprecipitated using rabbit anti-Phospho SMC3 (S1083) antibody orb1522621 at 6 µg per reaction.
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat, Sheep | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat, Sheep | |
Human | |
Rabbit | |
Polyclonal | |
Biotin |
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