SMC3, Phospho (S1083) Antibody

• Catalog Number: orb1522620
• Category: Antibodies
• Description: SMC3, Phospho (S1083) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Surrounding serine 1083
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:500 - 1:2,500; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SMC3, Phospho (S1083)
• UniProt ID: Q9UQE7
• NCBI: NP_005436.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: BAM; CSPG6; structural maintenance of chromosomes
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Phospho SMC3 (S1083) by western blot (WB). Samples: Whole cell lysate (50 µg) from HeLa cells treated with 100 µM etoposide and/or 100 µM phosphatase for 4 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho SMC3 (S1083) antibody orb1522620 used for WB at 1 µg/ml (upper panel) ; to detect total SMC3, rabbit anti-SMC3 was used for WB at 0.1 µg/ml (lower panel). Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) or 10 seconds (lower panel).

Detection of human Phospho SMC3 (S1083) by western blot of immunoprecipitates. Samples: Whole cell lysate (1mg for IP; 20% of IP loaded) from HeLa cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-). Antibodies: Phospho SMC3 (S1083) was immunoprecipitated using rabbit anti-Phospho SMC3 (S1083) antibody orb1522620 at 6 µg per reaction.