SMAD4 antibody

• Catalog Number: orb1151796
• Category: Antibodies
• Description: Rabbit monoclonal antibody to SMAD4
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: SMAD/6309R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: Recombinant human full-length SMAD4 protein (amino acids 1-552) was used as the immunogen for the recombinant SMAD4 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant SMAD4 antibody is available for research use only.
• UniProt ID: Q13485
• Storage: Aliquot the recombinant SMAD4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant SMAD4 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human small intestine with recombinant SMAD4 antibody (clone SMAD4/6309R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Immunohistochemical staining with recombinant SMAD4 antibody (clone SMAD4/6309R) showing loss of SMAD4 expression in pancreatic ductal adenocarcinoma and positive/retained SMAD4 staining in adjacent benign ductal epithelium and background stroma.