Smad3 (phospho-Thr179) antibody

• Catalog Number: orb769035
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Smad3 (phospho-Thr179)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Thr179. AA range:145-194
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 50
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-SMAD3 antibody, anti-MADH3 antibody, anti-Mot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of HELA cells using Phospho-Smad3 (T179) Polyclonal Antibody.

Immunohistochemistry analysis of paraffin-embedded human heart, using Smad3 (Phospho-Thr179) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells treated with TNF 20ng/ml 2', using Smad3 (Phospho-Thr179) Antibody. The lane on the right is blocked with the phospho peptide.