Smad3 (phospho-Ser208) antibody

• Catalog Number: orb6985
• Category: Antibodies
• Description: Smad3 (phospho-Ser208) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Ser208 (NL(p-S)PN)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 47 kDa
• Target: Phospho-Smad3 (Ser208)
• UniProt ID: P84022
• RRID: AB_10938499
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Smad3 (phospho S208); p-Smad3 (phospho S208); SMAD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry of A431.Primary Antibody (green line): Rabbit Anti-Phospho-Smad3 (Ser208) antibody; Dilution:1μg /10^6 cells.

Western blot:Lane 1:Huvec (Human) Cell;Lane 2:Hela (Human) Cell;Lane 3:HT1080 (Human) Cell;Lane 4:A431 (Human) Cell;Lane 5:A549 (Human) Cell;Primary:Anti-Phospho-Smad3 (Ser208) at 1:1000 dilution.