SMAD2/3 (phospho-T8) antibody

• Catalog Number: orb224172
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SMAD2/3 (Phospho-T8)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T8 of human SMAD2/3 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: SMAD2; SMAD3
• Entrez: 17126, 17127, 29357, 4088, 25631, 4087
• UniProt ID: Q62432, Q15796, P84025, O70436, Q8BUN5, P84022
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti SMAD2 antibody, anti MADH2 antibody, anti MAD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Bortolozzi, Roberta et al. Ribociclib, a Cdk4/Cdk6 kinase inhibitor, enhances glucocorticoid sensitivity in B-acute lymphoblastic leukemia (B-All) Biochem Pharmacol, 153, 230-241 (2018)

PubMed: 29408328

Western blot analysis of SMAD2/3 (Phospho-T8) expression in HEK293T (A), A375 (B), H460 (C), rat muscle (D), rat heart (E) whole cell lysates. (Predicted band size: 52; 48 kD; Observed band size: 60 kD)

Immunohistochemical analysis of SMAD2/3 (Phospho-T8) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.