Smad2 (phospho-Thr8) antibody

• Catalog Number: orb186235
• Category: Antibodies
• Description: Smad2 (phospho-Thr8) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Smad2/Smad3 around the phosphorylation site of Thr8 (PF(p-T)PP)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 52 kDa
• Target: phospho-Smad2/Smad3 (Thr8)
• UniProt ID: Q15796
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Smad2 + Smad3 (phospho T8); p-Smad2 + Smad3 (phosp
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry of Hela.Primary Antibody (green line): Rabbit Anti-phospho-Smad2/Smad3 (Thr8) antibody; Dilution:1μg /10^6 cells.