SMAD1/9 (phospho-S465/467) antibody

• Catalog Number: orb304592
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SMAD1/9 (phospho-S465/467)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S465/467 of human SMAD1/9 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: SMAD1; SMAD9
• Entrez: 4086, 17125
• UniProt ID: Q15797, P70340, O15198, P97588
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti SMAD1 antibody, anti BSP1 antibody, anti MADH
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SMAD1/9 (Phospho-S465/467) expression in HeLa (A) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 60 kD)

Immunohistochemical analysis of SMAD1/9 (Phospho-S465/467) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.