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Catalog Number | orb1247762 |
---|---|
Category | Antibodies |
Description | SLC7A11 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, IHC, WB |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-KGQTQNFKDAFSGRD |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50-55kDa band observed in lysates of cell lines A549 and U2OS, and in Human Smooth Muscle and Tonsil lysates (calculated MW of 55.4kDa according to NP_055146.1). Recommended concentration: 0.03-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Placenta and Kidney. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the vesicles/ cytoplasm of A549 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | SLC7A11 |
UniProt ID | Q9UPY5 |
NCBI | NP_055146.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | SLC7A11, solute carrier family 7, (cationic amino Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50-55kDa band observed in lysates of cell lines A549 and U2OS, and in Human Smooth Muscle and Tonsil lysates (calculated MW of 55.4kDa according to NP_055146.1). Recommended concentration: 0.03-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Placenta and Kidney. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the vesicles/ cytoplasm of A549 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1247762 (3.75 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1247762 (3.75 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
orb1247762 (0.3 ug/ml) staining of A549 (A) and U2OS (B) cell ysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1247762 (0.1 ug/ml) staining of Human Smooth Muscle (A) and Tonsil (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1247762 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing vesicle/cytoplasmic staining.
orb1247762 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Sheep | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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