SLC10A1 Rabbit Polyclonal Antibody

• Catalog Number: orb500786
• Category: Antibodies
• Description: SLC10A1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Canine, Equine, Mouse, Rabbit, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human NTCP (21-120/349aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 38 kDa
• Target: SLC10A1
• UniProt ID: Q14973
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Na+/taurocholate Cotransporting Polypeptide; Na(+)
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: 293T (blue). Primary Antibody: Rabbit Anti-SLC10A1 antibody (orb500786), dilution: 1 µg in 100 1 µl 1X PBS containing 0.5% BSA (green), Isotype Control Antibody: Rabbit IgG (orange) used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: K562 (human) cell Lysate at 40 ug, Primary: Anti-SLC10A1 (orb500786) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 38 kD, Observed band size: 48 kD.

Sample: Lane 1: Human HL60 cell lysates, Lane 2: Human K562 cell lysates, Lane 3: Human U-2 OS cell lysates, Primary: Anti-SLC10A1 (orb500786) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 38 kDa, Observed band size: 52 kDa.