Skp2 p45 rabbit pAb

• Catalog Number: orb766325
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Skp2 p45
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human SKP2/p45. AA range:366-415
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 47
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-SKP2 antibody, anti-FBXL1 antibody, anti-S-ph
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of Hela cell. 1, Skp2 p45 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). CYCS Monoclonal Antibody (4B10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western Blot analysis of various cells using Skp2 p45 Polyclonal Antibody diluted at 1:500.

Immunohistochemical analysis of paraffin-embedded Human Right kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human Right kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).