SKAP2 Antibody

• Catalog Number: orb1254394
• Category: Antibodies
• Description: SKAP2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SKAP2 (NP_003921.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 50kDa
• Target: SKAP2
• UniProt ID: O75563
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: SCAP2, src family associated phosphoprotein 2, MGC
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of mouse lung, using SKAP2 antibody (orb1254394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded mouse stomach using SKAP2 antibody (orb1254394) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat lung using SKAP2 antibody (orb1254394) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using SKAP2 antibody (orb1254394) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using SKAP2 antibody (orb1254394). Blue: DAPI for nuclear staining.