SIRT2 Antibody

• Catalog Number: orb1151614
• Category: Antibodies
• Description: Mouse monoclonal antibody to SIRT2
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-SIRT2-1A8
• Tested applications: FACS, IF, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Recombinant full-length human SIRT2/Sirtuin 2 protein was used as the immunogen for the Sirtuin 2 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Sirtuin 2 antibody is available for research use only.
• UniProt ID: Q8IXJ6
• Storage: Aliquot the Sirtuin 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Sirtuin 2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells using Sirtuin 2 antibody (green, clone PCRP-SIRT2-1A8) and phalloidin (red).

Immunofluorescent staining of PFA-fixed U-87 cells using Sirtuin 2 antibody (green, clone PCRP-SIRT2-1A8) and phalloidin (red).

FACS staining of PFA-fixed human MCF-7 cells with Sirtuin 2 antibody (blue, clone PCRP-SIRT2-1A8), and unstained cells (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Sirtuin 2 antibody (clone PCRP-SIRT2-1A8). These results demonstrate the foremost specificity of the PCRP-SIRT2-1A8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Sirtuin 2 antibody (clone PCRP-SIRT2-1A8) as confirmation of integrity and purity.