SIRT1 Antibody (C-term)

• Catalog Number: orb1926409
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB51254
• Tested applications: WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:2000, WB: 1:2000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 81681 Da
• Target: This SIRT1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 587-621 amino acids from the C-terminal region of human SIRT1.
• UniProt ID: Q96EB6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: NAD-dependent protein deacetylase sirtuin-1, hSIRT
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-SIRT1 Antibody (C-term) at 1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 82 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-SIRT1 Antibody (C-term) at 1:1000 dilution. Lane 1: NIH/3T3 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: MDA-MB-231 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 120 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-SIRT1 Antibody (C-term) at 1:2000 dilution. Lane 1: K562 whole cell lysates. Lane 2: Hela whole cell lysates. Lane 3: A549 whole cell lysates. Lane 4: human testis lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 82 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.