SIGLEC8 Antibody (PE)

• Catalog Number: orb1147807
• Category: Antibodies
• Description: SIGLEC8 is a sialic acid binding lectin similar to CD33. In its cytoplasmic comain it contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), and a motive similar to a binding site for SLAM-associated protein. SIGLEC8 is expressed e.g. in lymph nodes and spleen. It is an eosinophil marker, although it can be found also on the surface of mast cells. Crosslinking of SIGLEC8 leads to apoptosis. Soluble form of SIGLEC8 can be foud in human serum, especially in case of eosinophil-associated diseases.
• Clonality: Monoclonal
• Clone Number: 7C9
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Extracellular domain of human SIGLEC8 fused with Fc fragment of human IgG1
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: SIGLEC8
• Entrez: 27181
• UniProt ID: Q9NYZ4
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: SAF2
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Separation of human SIGLEC8 positive eosinophil granulocytes (red-filled) from eosinophil granulocytes (black-dashed) stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 1.7 μg/ml, same as SIGLEC8 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human SIGLEC8 (7C9) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).