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Catalog Number | orb1238667 |
---|---|
Category | Antibodies |
Description | SIGLEC15 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Anti-SIGLEC15 antibody (orb1238667) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human SIGLEC15. The immunogen is located within amino acids 270-320 of SIGLEC15. |
Concentration | 1 mg/mL |
Dilution range | WB: 2 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 36kDObserved: 34-36kD |
Target | SIGLEC15 |
UniProt ID | Q6ZMC9 |
NCBI | NP_998767 |
Storage | SIGLEC15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | SIGLEC15 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | SIGLEC15 Antibody: CD33L3, HsT1361, SIGLEC-15, CD3 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: SIGLEC15, orb1238667 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Tissues. Loading: 15 µg of lysates per lane. Antibodies: SIGLEC15, orb1238667 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: SIGLEC15, orb1238667 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: SIGLEC15, orb1238667 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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