SIAH Interacting Protein/CACYBP Antibody

• Catalog Number: orb76272
• Category: Antibodies
• Description: SIAH Interacting Protein/CACYBP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human CACYBP (160-175aa NTRWDYLTQVEKECKE), identical to the related rat and mouse sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, RatWestern blot, 0.1-0.5μg/ml, Human, Rat, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 26210 MW
• UniProt ID: Q9HB71
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Calcyclin-binding protein;CacyBP;hCacyBP;S100A6-bi
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: Rat Liver Tissue Lysate, Lane 2: Rat Brain Tissue Lysate, Lane 3: Rat Spleen Tissue Lysate, Lane 4: SMMC Cell Lysate, Lane 5: COLO320 Cell Lysate, Lane 6: SW620 Cell Lysate, Lane 7: 293T Cell Lysate using CACYBP antibody

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