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Sheep IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
Catalog Number: orb347933
| Catalog Number | orb347933 |
|---|---|
| Category | Antibodies |
| Description | Sheep IgG (H&L) antibody (Alkaline Phosphatase) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC, WB |
| Reactivity | Sheep |
| Isotype | IgG |
| Immunogen | Sheep IgG whole molecule |
| Concentration | 0.5 mg/mL |
| Dilution range | ELISA: 1:1,000 - 1:5,000, IHC: 1:200 - 1:1,000, WB: 1:400 - 1:1,600 |
| Form/Appearance | Liquid (sterile filtered) |
| Purity | Secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against Human Serum Proteins. |
| Conjugation | AP |
| Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
| Buffer/Preservatives | 0.09% (w/v) Sodium Azide |
| Alternative names | Rabbit Anti-Sheep IgG alkaline phosphatase Conjuga Read more... |
| Note | For research use only |
| Application notes | Secondary antibody conjugated to alkaline phosphatase is available in a variety of formats. Conjugated Secondary Antibody is suitable for western blot, ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency. |
| Expiration Date | 12 months from date of receipt. |
