Sheep IgG (H&L) antibody (Texas Red)

• Catalog Number: orb347962
• Category: Antibodies
• Description: Sheep IgG (H&L) antibody (Texas Red)
• Species/Host: Donkey
• Clonality: Polyclonal
• Tested applications: FC, FLISA, IF, WB
• Reactivity: Sheep
• Isotype: IgG
• Immunogen: Sheep IgG whole molecule
• Concentration: 2.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Sheep IgG (H&L) Antibody Texas Red Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum.
• Conjugation: Texas Red
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Donkey Anti-Sheep IgG Texas Red Conjugated Antibod
• Note: For research use only
• Application notes: Sheep IgG (H&L) Antibody Texas Red Conjugated antibody has been tested by western blot and are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Western Blot of Donkey anti-Sheep IgG Texas Red Conjugated Antibody. Lane 1: Sheep IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red donkey secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Sheep IgG. Other band(s): None.