Sheep IgG (H&L) Antibody Pre-Adsorbed

• Catalog Number: orb347915
• Category: Antibodies
• Description: Sheep IgG (H&L) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Sheep
• Isotype: IgG
• Immunogen: Sheep IgG whole molecule
• Concentration: 1.4 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against Human Serum Proteins.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Sheep IgG pre-adsorbed antibody
• Note: For research use only
• Application notes: Anti-Sheep IgG antibody has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Rabbit Anti-Sheep IgG Antibody (Min X Human Serum Proteins) tested against purified Sheep IgG MX Hu. Each well was coated in duplicate with 1.0 µg of Sheep IgG (p/n orb2652741) [Green Line] and Human IgG (p/n orb346219) [Blue Line]. The working dilution of Sheep IgG MX Hu is 1:71000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit IgG HRP conjugated (p/n orb347654) and TMB substrate (p/n orb348651).