Sheep IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

• Catalog Number: orb347927
• Category: Antibodies
• Description: Sheep IgG (H&L) antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Sheep
• Isotype: IgG
• Immunogen: Sheep IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:22,000, IHC: 1:300 - 1:1,500, WB: 1:500 - 1:3,000
• Form/Appearance: Lyophilized
• Purity: Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against Human Serum Proteins.
• Conjugation: HRP
• Storage: Store secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: Rabbit Anti-Sheep IgG peroxidase Conjugated Antibo
• Note: For research use only
• Application notes: Anti-Sheep IgG Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Rabbit Anti-Sheep IgG Antibody Peroxidase Conjugate (Min X Human Serum Proteins) tested against purified Sheep IgG Min X Hu HRP. Each well was coated in duplicate with 1.0 µg of Sheep IgG (p/n orb2652741). The working dilution is 1:57000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.