Sheep IgG (H&L) Antibody

• Catalog Number: orb347912
• Category: Antibodies
• Description: Sheep IgG (H&L) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Sheep
• Isotype: IgG
• Immunogen: Sheep IgG whole molecule
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:50,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG and Sheep Serum.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rabbit Anti-Sheep IgG Antibody, Rabbit Anti Sheep
• Note: For research use only
• Application notes: Anti-Sheep IgG antibody has been tested by ELISA and is suitable for use in ELISA, lateral flow, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

ELISA results of Rabbit Anti-Sheep IgG Antibody tested against purified Sheep IgG protein. Each well was coated in duplicate with 10 µg of Sheep IgG (p/n orb2652741). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1:60800. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 1% fish gelatin as blocking buffer, Goat Anti-Rabbit IgG Peroxidase (p/n orb347654), and TMB substrate p/n orb348651.