Sheep IgG Fc Antibody

• Catalog Number: orb347913
• Category: Antibodies
• Description: Sheep IgG F(c) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Sheep
• Isotype: IgG
• Immunogen: Anti-Sheep IgG was produced by repeated immunization with sheep IgG F(c) fragment in rabbit.
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:200,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Sheep IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum. No reaction was observed against Sheep IgG F(ab).
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rabbit Anti-Sheep IgG F(c) Antibody, Rabbit Anti-S
• Note: For research use only
• Application notes: Anti-Sheep IgG F(c) antibody has been tested by ELISA and dot blot and is assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Rabbit Anti-Sheep IgG F(c) Antibody tested against purified Sheep IgG F(c). Each well was coated in duplicate with 1.0 µg of Sheep IgG F(c) (p/n orb346368) [Red Line] and Sheep IgG [Green Line]. The working dilution of Sheep IgG F(c) is 1:45000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, Goat Anti-Rabbit IgG HRP conjugated (p/n orb347654) and TMB substrate (p/n orb348651).