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Catalog Number | orb346368 |
---|---|
Category | Proteins |
Description | Sheep IgG F(c) Antibody |
Tested applications | SDS-PAGE, WB |
Isotype | IgG F(c) |
Concentration | 1.0 mg/mL |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum, anti-Sheep IgG and anti-Sheep IgG F(c). No reaction was observed against anti-Sheep IgG F(ab’)2 or anti-Papain. |
Conjugation | Unconjugated |
Source | Sheep |
Biological Origin | Sheep |
Storage | Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Sheep IgG F(c) Fragment Read more... |
Note | For research use only |
Application notes | Sheep IgG F(c) has been tested by SDS-Page and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
Expiration Date | 12 months from date of receipt. |
DOT, IF | |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Sheep IgG, anti-Sheep IgG F(c) and anti-Sheep Serum. No reaction was observed against anti-Sheep IgG F(ab’)2 or anti-Papain. | |
Sheep |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep IgG, anti-Sheep IgG F(c) and anti-Sheep Serum. No reaction was observed against anti-Sheep IgG F(ab’)2 or anti-Papain. | |
Sheep |
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