Sheep IgG F(ab')2 Antibody Peroxidase Conjugated

• Catalog Number: orb347926
• Category: Antibodies
• Description: Sheep IgG F(ab')2 antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Sheep
• Isotype: IgG
• Immunogen: Sheep IgG F(ab')2 fragment
• Concentration: 1.5 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab’)2 and Sheep Serum. No reaction was observed against Sheep IgG F(c).
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: Rabbit Anti-Sheep IgG F(ab')2 peroxidase Conjugate
• Note: For research use only
• Application notes: This product has been assayed against 1.0 µg of Sheep IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry results using rabbit anti-sheep IgG F(ab')2 HRP. Antigen retrieval was performed by heating the paraffin sections in an autoclave at 121°C for 15 min. The paraffin sections were then treated with 10% normal sheep serum for staining against anti-human TSLP antibody. After several washes with PBS, the sections were stained with Peroxidase-conjugated affinity-purified anti-sheep IgG F(ab')2 secondary antibody for 1.5 h. The samples were treated with 0.3% H2O2 in cold methanol for 30 min. Finally, 0.1 mg/ml 3, 3'-diaminobenzidine tetrahydrochloride was applied to sections for 10 min. The sections were counterstained with hematoxylin. (a, b) Immunohistochemical localization of Lag-positive cells in Hassal's corpuscles of the thymus at 5 months (a, brown) and 8 months (b, dark blue) of human ontogeny. (c) Immunohistochemical localization of TSLP-R (brown, arrowheads) in Hassal's corpuscles of the thymus at 5 months of gestation. Bars 10 µm.