Sheep IgG Antibody

• Catalog Number: orb346367
• Category: Proteins
• Description: Sheep IgG Antibody
• Tested applications: ELISA, IHC, SDS-PAGE, WB
• Isotype: IgG
• Concentration: 10.0 mg/mL
• Dilution range: ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
• Form/Appearance: Lyophilized
• Purity: Sheep IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Sheep IgG whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep IgG and anti-Sheep Serum.
• Conjugation: Unconjugated
• Source: Sheep
• Biological Origin: Sheep
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Sheep IgG whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: Sheep Immunoglobulin Gamma, Immunoglobulin G
• Note: For research use only
• Application notes: Sheep IgG whole molecule has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
• Expiration Date: 12 months from date of receipt.

SDS PAGE Results of Sheep IgG Whole Molecule. Lane 1: Sheep IgG Reduced [5 µg]. Lane 2: Sheep IgG Reduced [1 µg]. Lane 3: Opal Prestained Molecular Weight Marker. Lane 4: Sheep IgG Non-Reduced [1 µg]. Lane 5: Sheep IgG Non-Reduced [5 µg]. 4-20% Gel, Coomassie stained.

SDS-Page of Sheep IgG Lane 1: Sheep IgG Non-Reduced Lane 2: Sheep IgG Reduced Load: 1.0 ug per lane Non-Reduced Predicted/Obsevered Size: 160 kDa/160 kDa Reduced Predicted/Obsevered Size: 28 and 55 kDa/28 and 55 kDa.