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Catalog Number | orb97023 |
---|---|
Category | Antibodies |
Description | SHC/SHC1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IHC, IHC-Fr, WB |
Predicted Reactivity | Hamster |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SHC (424-440aa DPSYVNVQNLDKARQAV), different from the related rat and mouse sequences by two amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 62822 MW |
UniProt ID | P29353 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | SHC-transforming protein 1;SHC-transforming protei Read more... |
Note | For research use only |
Application notes | WB: The detection limit for SHC1 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Intestine Tissue using SHC1 antibody
Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Brain Tissue using SHC1 antibody
Western blot analysis of Lane 1: Recombinant Human SHC1 Protein 10ng, Lane 2: Recombinant Human SHC1 Protein 5ng, Lane 3: Recombinant Human SHC1 Protein 2.5ng using ERBB4 antibodySHC1 antibody
Western blot analysis of Lane 1: Rat Brain Tissue Lysate, Lane 2: A549 Cell Lysate, Lane 3: A431 Cell Lysate, Lane 4: 293T Cell Lysate, Lane 5: HELA Cell Lysate, Lane 6: JURKAT Cell Lysate using SHC1 antibody
Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Brain Tissue using SHC1 antibody
Immunohistochemical analysis of formalin-fixed paraffin embedded Human Lung Cancer Tissue using SHC1 antibody
IHC, IHC-Fr, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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