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Catalog Number | orb251574 |
---|---|
Category | Antibodies |
Description | SHC/SHC1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IHC-Fr, WB |
Predicted Reactivity | Hamster |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SHC (536-564aa VDPEGVVRTKDHRFESVSHLISYHMDNHL), identical to the related mouse and rat sequences. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 62822 MW |
UniProt ID | P29353 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | SHC-transforming protein 1;SHC-transforming protei Read more... |
Note | For research use only |
Application notes | WB: The detection limit for SHC is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of SHC using anti-SHC antibody.Lane 1:human A431 Cell;2:human HeLa Cell;3:human HepG2 Cell;4:human Jurkat Cell;5:human K562 Cell;6:human THP-1 Cell;7:rat C6 Cell;8:mouse thymus Tissue;9:mouse RAW246.7 Cell;10:mouse NIH3T3 Cell.
IHC analysis of SHC1 using anti-SHC1 antibody.SHC1 was detected in paraffin-embedded section of Mouse Brain Tissue.
IHC analysis of SHC1 using anti-SHC1 antibody.SHC1 was detected in paraffin-embedded section of Rat Brain Tissue.
IHC analysis of SHC1 using anti-SHC1 antibody.SHC1 was detected in paraffin-embedded section of Human Lung Cancer Tissue.
IHC analysis of SHC1 using anti-SHC1 antibody.SHC1 was detected in frozen section of Human Placenta Tissue.
ICC, IHC, IHC-Fr, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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