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Catalog Number | orb764329 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Shc (phospho-Tyr427) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human Shc around the phosphorylation site of Tyr427. AA range:393-442 |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
MW | 66(p66 isoform), 52(p52 isoform), 46(p46 isoform) |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-SHC1 antibody, anti-SHC antibody, anti-SHCA a Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot analysis of various cells using Phospho-Shc (Y427) Polyclonal Antibody diluted at 1:500.
Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using Shc (Phospho-Tyr427) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 5', using Shc (Phospho-Tyr427) Antibody. The lane on the right is blocked with the phospho peptide.
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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