SF3B3 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1499323
• Category: Antibodies
• Description: SF3B3 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: R7F1
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human, Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human SF3B3
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-1:400, IF=1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 136 kDa
• Target: SF3B3
• UniProt ID: Q15393
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: KIAA0017; Pre mRNA splicing factor SF3b 130 kDa su
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: Jurkat, 3: HEK-293, 4: RAW 264.7, 5: C6, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 135 kDa, Observed MW: 135 kDa.

Tissue: Mouse colon, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:2000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499323.

Tissue: Rat cerebrum, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499323.