SETD7 Antibody [RAB-C220], Rabbit IgG

• Catalog Number: orb1671529
• Category: Antibodies
• Description: SETD7 Antibody [RAB-C220], Rabbit IgG
• Clonality: Recombinant
• Clone Number: RAB-C220
• Tested applications: ChIP, ELISA, FC, IF
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing SETD7 protein under non-denaturing conditions.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: SETD7
• UniProt ID: Q8WTS6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: SET7/9, Histone-lysine N-methyltransferase SETD7,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-SETD7 antibody R Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Western Blot using anti-SETD7 antibody R Nuclear lysate of MCF7(A) (0.01 ug/ml)- Jurkat(B) (0.1 ug/ml) cells and A549(C) (0.03 ug/ml) cell lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescence staining of HeLa cells with anti-SETD7. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671529- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.