SERPINB5 Antibody / MASPIN

• Catalog Number: orb2635757
• Category: Antibodies
• Description: Maspin/SERPINB5 is structurally a serine protease inhibitor (serpin) that was initially isolated from normal human mammary epithelial cells. Serpins are a family of proteins that inhibit Chymotrypsin-like serine proteinases. Serpins control activated proteinases and several are involved in the regulation of cell death. Maspin is found in the extracellular matrix and at the plasma membrane. Maspin has been shown to act at the cell surface to block cell motility and inhibit invasion of breast and prostate cancer cells. Maspin is present in normal mammary epithelial cells but is absent in many tumor cell lines, yet no major structural alterations of the Maspin gene have been identified in tumor cells. Similarly, Maspin is expressed in normal prostate cells and downregulated or absent in prostate tumor cells.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SERPINB5/4972
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A portion of amino acids1-200 was used as the immunogen for the SERPINB5 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This SERPINB5 antibody is available for research use only.
• UniProt ID: P36952
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the SERPINB5 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human skin with SERPINB5 antibody (clone SERPINB5/4972). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human skin tissue with SERPINB5 antibody (clone SERPINB5/4972). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human PC3 cell lysate using SERPINB5 antibody (clone SERPINB5/4972). Predicted molecular weight: 42~45 kDa.

Western blot testing of human SW732 cell lysate using SERPINB5 antibody (clone SERPINB5/4972). Predicted molecular weight: 42~45 kDa.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using SERPINB5 antibody (clone SERPINB5/4972). These results demonstrate the foremost specificity of the SERPINB5/4972 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.