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Catalog Number | orb1248235 |
---|---|
Category | Antibodies |
Description | SERPINB1 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-KTINQWVKGQTEGK |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 42-45kDa bands observed in Human Placenta lysates (calculated MW of 42.7kDa according to NP_109591.1). The fainter 45kDa band likely represents the acetylated form. Both bands are blocked by the immunizing peptide. Recommended concentration: 0.5-2ug/ml.Immunohistochemistry:Paraffin embedded Human Spleen (neutrophils). Recommended concentration: 5ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Approx 42-45kDa |
Target | SERPINB1 |
UniProt ID | P30740 |
NCBI | NP_109591.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | SERPINB1, serpin peptidase inhibitor, clade B (ova Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 42-45kDa bands observed in Human Placenta lysates (calculated MW of 42.7kDa according to NP_109591.1). The fainter 45kDa band likely represents the acetylated form. Both bands are blocked by the immunizing peptide. Recommended concentration: 0.5-2ug/ml.Immunohistochemistry:Paraffin embedded Human Spleen (neutrophils). Recommended concentration: 5ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1248235 (0.5 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
orb1248235 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, IF, IHC, IP, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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