SEMA3A antibody

• Catalog Number: orb537478
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SEMA3A
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human Semaphorin 3A (NP_006071.1).
• Dilution range: WB: 1:500-1:1000
• Conjugation: Unconjugated
• MW: 89kDa
• UniProt ID: Q14563
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Alternative names: SEMA3A, COLL1, HH16, Hsema-I, Hsema-III, SEMA1, SE
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from mouse brain, using Semaphorin 3A Rabbit pAb (orb537478) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.