SDHB Antibody / Succinate dehydrogenase B

• Catalog Number: orb1824552
• Category: Antibodies
• Description: Succinate dehydrogenase B (SDHB) is an iron-sulfur subunit of mitochondrial complex II, a key component of the citric acid cycle and the electron transport chain, a respiratory complex that catalyzes the oxidation of succinate in the mitochondrial membrane.Many cancers are generally positive for SDHB, including renal cell carcinomas and gastrointestinal stromal tumors. A subset of RCC and GIST tumors that are associated with SDH mutations, Carney-Stratakis Syndrome or Carney Triad exhibit a loss of SDHB expression.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: SDHB/8974R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A synthetic peptide corresponding to human SDHB protein was used as the immunogen for the Succinate dehydrogenase B antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Succinate dehydrogenase B antibody is available for research use only.
• UniProt ID: P21912
• Storage: Aliquot the Succinate dehydrogenase B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human hepatocellular carcinoma tissue with Succinate dehydrogenase B antibody (clone SDHB/8974R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.